PREPARATION AND CHARACTERIZATION OF NUCLEOSIDE ANALOGUES AND STUDYING THEIR INHIBITION EFFECT ON GOT ENZYME

This article is divided into two parts. The first part focuses on the preparation of new Mannich derivatives of nucleoside cytidine. To obtain these derivatives, it is required the interaction of cytidine with secondary amines (morpholine, pyrazine, pyridine and pyrrolidine) in the presence of formaldehyde. Hence, nucleoside analogues (1, 2, 3 and 4) are obtained. Then, the prepared compounds are characterized and confirmed using 13C.N.M.R, 1H.N.M.R, FT-IR techniques. On the other hand, the second part examined the effect of these compounds on glutamate oxaloacetate transaminase (GOT) in serum samples of a normal human. This is performed by measuring the enzyme activity (with and without the compounds mentioned above) as inhibitors under the same conditions. Furthermore, the inhibition ratio has been calculated, determine inhibitors with the highest rate of inhibition, and define the inhibition type as well. The study findings reveal that these compounds have a significant impact as inhibitors of GOT enzyme activity in human serum.