Nucellar Embryogenesis and Artificial Seed Production in Citrus reticulata.

A study was undertaken to develop a protocol for nucellar embryogenesis and artificial seed preparation in Khasimandarin. Nucellar embryoids were formed in 35 days after culturing in NE4 media containing MT basal with malt extract (500mg l-1) (W/V)BAP( 0.5 mg l-1). These nucellar embryoids were used in preparation of artificial seed. Upon reculture, the artificial seed differentiated in to whole plants in 100-125 days in modified MT media containing in Malt extract (500 mg l-1) and BAP (0.25 mg l-1). Storage observations revealed that artificial seeds can be stored at lower temp (4 C) without altering their viability.