PREPARATION AND CHARACTERIZATION OF SILVER NANOPARTICLES BY Justicia adhotoda AND Ocimum sanctum FORMULATION USING TRANSMISSION ELECTRON MICRSCOPY

Biological synthesis involves the reduction of stabilization of metal ions using different types of organisms. Plants are a very good source for silver nanoparticles (Ag Nps) because they have a wide range of strong reducing potential. Synthesis of silver nanoparticles using plant extracts like Justicia adhatoda and Ocimum sanctum (tulsi) mixture shows that biological synthesis via an in vitro method is most applicable, highly economical and eco-friendly. The present study describes that biosynthesis of silver nanoparticles by using the plant extract of Justicia adhatoda and Ocimum sanctum in an in vitro condition and its characterization using TEM.

Aim: This study aims at the preparation and characterization of silver nanoparticles produced using Justicia adhotoda and Ocimum sanctum herbal formulation using TEM.

Materials and Methods: The main method used in synthesizing these nanoparticles is known as Green Synthesis, using the plant extract of Ocimum sanctum and Justicia adhatoda and then characterization by UV-Visible spectroscopy and TEM.

Results: The surface resonance was found to be 10-35 nm, which conforms to the formation of the silver nanoparticles. TEM images revealed relatively hexagonal shapes which confirmed the formation of silver nanoparticles. AgNps have many shapes, which may be spherical and triangle shaped, the size of the nanoparticles is normally around 10-60 nm. The silver nanoparticles were crystalline in nature, with UV absorbance of around 320 nm.

Conclusion: The characterisation of the silver nanoparticles with TEM enables future research to analyse the antibacterial, antifungal, antioxidant, cytotoxic, and antiinflammatory properties of this formulation. This novel formulation might provide a fresh insight into the various applications in the biomedical field.