The Use of Streptomyces capoamus Isolated from Caatinga in the Production and Characterization of Microbial Endoglucanase

Cellulases are hydrolases of great importance to industries, especially due to their ability to produce
ethanol via hydrolysis of cellulolytic materials. Actinomycetes are the producers of these enzymes,
particularly the genus Streptomyces sp. The present study is the first report on the production and
characterization of cellulolytic complex secreted by Streptomyces capoamus, isolated from the
rhizosphere soil of Caatinga. In selecting the microbial producers of cellulolytic complex in qualitative
tests, 171 x micro organism showed the most expressive enzymatic index. Regarding the production
time of the complex, fermentation was done for 7 days, with aliquots being taken every 24 h. Peak
production was obtained during 48 h fermentation. It was done at 37°C and under an agitation of 180
rpm. It was noted also that the 171x micro-organism produced the enzyme in greater quantity. The
experiment was done with the most significant actinomycetes (171x), optimal substrate concentration
(carboximeticellulose), cultivation temperature and pH of initial output. The results showed that a
higher cellulolytic complex was obtained with 2% substrate, 45°C temperature and initial pH 4.0. The
microorganism was identified at genus level by microculture method; and with molecular identification
method, it was identified as S. capoamus UFPEDA-3410. In optimal culture conditions, this strain
produced 0.309 U/mL cellulose, a good production for a thermostable endoglucanase stable in a
broad range of pH and stable temperature. It has potential applications in a wide range of industries.
Industrial processes are generally carried out at elevated temperatures. Therefore enzymes with a
high optima temperature and stability are desired for such applications.