The Positive Effect of Lithium for Elevated Specific Activities of Alkaline Phosphatase and β-Galactosidase on the Liver and Brain Cell Membrane Integrity in Young and Old Aged Rats

Objectives: Differentiate the particular activity of Alkaline Phosphatase and -Galactosidase in LiCl2 treated young and old aged rat's brain and liver to their control. Materials and Methods: For 10 and 20 days, four groups of male wistar rats (each n=5) were used to assess the levels of Alkaline phosphatase and -galactosidase. In comparison to the control, LiCl2 treated the brain and liver of young and old rats. Results: LiCl2 increases protein content, which is required for old brain cell activation. Alkaline phosphatase and -Galactosidase showed increased specific activity in the brain and liver of young and old rats treated with LiCl2 for 10 and 20 days, respectively, when compared to their control. In fact Alkaline phosphatase showed significantly higher activity for 10 and 20 days LiCl2 treated young brain exhibited 16.77 ± 0.309 and 27.74 ± 0.237 respectively as compared to their control 3.80 ± 0.230 while old rat's brain exhibited 14.28 ± 0.215 and 16.30 ± 0.24 respectively as compared to their control 8.65 ± 0.304 nm/min/mg of protein, similarly higher activity obtained for young liver 19.51 ±0.487 and 21.28 ± 0.317 as compared to their control 3.67 ± 0.402 and finally the old liver exhibited 15.02 ± 0.355 and 18.45 ± 0.439 as compared to their control 3.26 ± 0.304 nm/min/mg of protein respectively. Conclusion: These findings confirm that both membrane bound Alkaline Phosphatase and -Galactosidase enzymes exhibited higher specific activity for LiCl2 treatment in wistar rats brain and liver cells. So lithium has a positive effect on both young and old brain cells for -galactosidase while having a greater positive effect on Alkaline phosphatase in the rat liver and brain for cell membrane integrity.